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The patient was a muscular man with no abnormalities on physical examination

Ye et al., 2006)

Cycling was performed using an Eppendorf Mastercycler (Hamburg, Germany), as follows: a pre-PCR step of a 5-min denaturation at 94C, followed by 30 cycles of denaturation at 94C for 30 sec, annealing for 30 sec and extension at 72C for 30 sec and a final 5-min incubation at 72C